Journal: Proteome Science

Article Title: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

doi: 10.1186/1477-5956-2-9

Figure Lengend Snippet: Schematic layout of antibody microarray slide and RCA immunoassay. At the far left is an illustration of the 1" × 3" slide platform containing sixteen individual sample wells with an etched barcode. Within each of the wells, a 16 × 16 configuration of printed capture antibodies is arrayed. Each of the capture antibodies is capable of binding analytes from applied samples and undergoing RCA signal amplification. Finally, the fluorescently labeled signal, detected through conventional laser scanning, is quantified.

Article Snippet: The manual RCA microarray immunoassay reported previously was modified to optimize performance on an automated platform (Protedyne BioCube).

Techniques: Microarray, Binding Assay, Amplification, Labeling

Journal: Proteome Science

Article Title: Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

doi: 10.1186/1477-5956-2-9

Figure Lengend Snippet: An example of raw data quality and outlier removal. (panel a, top) Raw data (37 analytes) from array 4 containing all sample replicates shown on an MvA plot (a typical microarray data plot of the log ratio vs. the log difference for each pair of intensities. See: Dudoit, S., Yang, Y. H, Callow, M. J., and Speed, T. P. (2002) Statistica Sinica 12 , 111–140). The dashed lines indicate a 99% confidence interval around the data and outliers of this interval are shown in red, black or magenta. (panel b, bottom) Redacted data with 1% of outlier data removed (all points outside of the displayed confidence interval).

Article Snippet: The manual RCA microarray immunoassay reported previously was modified to optimize performance on an automated platform (Protedyne BioCube).

Techniques: Microarray

Journal: Clinical Chemistry

Article Title: Protein Microarrays for Personalized Medicine

doi: 10.1373/clinchem.2009.137158

Figure Lengend Snippet: Work flow of protein microarray applications for biomarker discovery and their application in personalized medicine.

Article Snippet: GenTel BioSciences , APiX and PANDEIA antibody microarray multiplexed immunoassays , Comparative protein profiling.

Techniques: Microarray, Biomarker Discovery

Journal: Clinical Chemistry

Article Title: Protein Microarrays for Personalized Medicine

doi: 10.1373/clinchem.2009.137158

Figure Lengend Snippet: Commercially available miniaturized and parallelized immunoassays based on planar microarray platforms.

Article Snippet: GenTel BioSciences , APiX and PANDEIA antibody microarray multiplexed immunoassays , Comparative protein profiling.

Techniques: Microarray, Phospho-proteomics, Transduction, Protein-Protein interactions, Ab Array, Enzyme-linked Immunosorbent Assay

Journal: Clinical Chemistry

Article Title: Protein Microarrays for Personalized Medicine

doi: 10.1373/clinchem.2009.137158

Figure Lengend Snippet: Characteristics of the different protein microarrays.

Article Snippet: GenTel BioSciences , APiX and PANDEIA antibody microarray multiplexed immunoassays , Comparative protein profiling.

Techniques: Microarray, Antibody Labeling

Journal: Annals of Neurology

Article Title: Antibody Response to HML ‐2 May Be Protective in Amyotrophic Lateral Sclerosis

doi: 10.1002/ana.26466

Figure Lengend Snippet: List of HERV‐K (HML‐2) Sequences Encoded in the Human Genome Aligning with the Peptide Used in the ELISA Assay

Article Snippet: Epitope mapping of the antibodies to HML‐2 env was performed by using peptide microarrays covering the complete sequence of the protein (Uniprot ID: Q69384) (PEPperCHIP Immunoassay, PepperPrint, Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: BDNF expression in adult Bdnf cKO mice at an early time point after BDNF gene deletion.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: miR-1 is downregulated in DRG of adult Bdnf cKO mice at an early time point after BDNF gene deletion. (A) Expression of miR-1 and (B) miR-124a (control) in DRG were detected using qRT-PCR. Data are presented as normalized levels (n = 9). White bars represent data from WT mice, while black bars represent data from BDNFAvCreERT2 mice 10 days after the last tamoxifen injection. Data are presented as mean ± SEM. Statistical analysis was performed with unpaired t-test; *p < 0.05.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing, Control, Quantitative RT-PCR, Injection

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: Hsp60 and Cx43 expression in DRG of adult Bdnf cKO animals at an early time point after BDNF gene deletion (A) Hsp60 and (B) Cx43 protein expression in DRG were determined by Western blot analysis. Data are presented as normalized levels (n = 8). White bars represent data from WT mice, while black bars represent data from BDNFAvCreERT2 mice 10 days after the last tamoxifen injection. Data are presented as mean ± SEM. Statistical analysis was performed with unpaired t-test; *p < 0.05.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing, Western Blot, Injection

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: BDNF expression in DRG of adult Bdnf cKO mice at a late time point after BDNF gene deletion. Quantification of BDNF mRNA in DRG with qRT-PCR, presented as normalized levels (n = 5). White bars represent data from WT mice, while black bars represent data from BDNFAvCreERT2 mice 32 days after the last tamoxifen injection. Data are presented as mean ± SEM. Statistical analysis was performed with unpaired t-test; *p < 0.05.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing, Quantitative RT-PCR, Injection

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: MiR-1 expression changes in DRG of adult Bdnf cKO mice at a late time point after BDNF gene deletion. Expression of miR-1 in DRG using qRT-PCR. Data are presented as normalized levels (n = 5). White bars represent data from WT mice, while black bars represent data from Bdnf cKO mice 32 days after the last tamoxifen injection. Data are presented as mean ± SEM. Statistical analysis was performed with unpaired t-test.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing, Quantitative RT-PCR, Injection

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: Hsp60 and Cx43 expression in DRG of adult Bdnf cKO animals at a late time point after BDNF gene deletion. Hsp60 (A) and Cx43 (B) protein expression in DRG were determined by Western blot analyses. Data are presented as normalized levels (n = 5). White bars represent data from WT mice, while black bars represent data from Bdnf cKO mice 32 days after the last tamoxifen injection. Data are presented as mean ± SEM. Statistical analysis was performed with unpaired t-test.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Expressing, Western Blot, Injection

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: MiRNA microarray in DRG of adult Bdnf cKO mice at a late time point after BDNF gene deletion. (A) Hierarchical clustering of differentially expressed microRNAs. 167 miRNAs were significantly dysregulated in DRG of late targeted adult Bdnf cKO mice (p < 0.05). Signal intensities (log2, normalized) are color coded: Green denotes downregulation while red denotes upregulation relative to the median. Statistical analysis was performed using one-way-ANOVA. (B) 54 differentially expressed miRNAs in DRG of late targeted adult Bdnf cKO mice. The shown data required a minimum miRNA expression change of 20% compared to WT mice and are grouped by statistical significance (*p < 0.05; **p < 0.01). Statistical analysis was performed with one-way ANOVA.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques: Microarray, Expressing

Journal: Molecular and Cellular Neurosciences

Article Title: MicroRNA-1-associated effects of neuron-specific brain-derived neurotrophic factor gene deletion in dorsal root ganglia

doi: 10.1016/j.mcn.2016.06.003

Figure Lengend Snippet: Model of miR-1-associated effects in DRG-specific conditional BDNF-deficient mice. (A) miR-1 negatively regulates BDNF and the neuropathic pain-associated proteins Hsp60 and Cx43. Hypothesized positive regulatory loop from BDNF towards miR-1. (B) Hypothesized impairment of the positive feedback loop due to BDNF gene targeting.

Article Snippet: BDNF protein levels of DRG were analyzed using the BDNF Emax® ImmunoAssay System (Promega, Madison, Wisconsin).

Techniques:

Journal: Advanced healthcare materials

Article Title: Magnet Patterned Superparamagnetic Fe 3 O 4 /Au Core-Shell Nanoplasmonic Sensing Array for Label-Free High Throughput Cytokine Immunoassay

doi: 10.1002/adhm.201801478

Figure Lengend Snippet: (a) Illustrations of the magnet assisted patterning process of the FACSNP microarray. (b) Dark-field microscopy images of the FACSNP microarray on the glass substrate. The FACSNPs confined in PDMS microwells self-assembled into a series of regular square-shape sensing spot arrays with the assistance of the external magnetic field. (c) After the patterning process, the FACSNP microarrays were functioned with four different antibodies in defined sensing areas for multiplex detection of four cytokines. (d) Dark-field microscopy image of individual FACSNPs biosensing spot at higher magnification, showing the well dispersed FACSNPs immobilized in the sensing spot. (e) SEM image of the FACSNPs before antibody function and (f) after successful antibody attachment.

Article Snippet: As such, the FACSNP microarray immunoassay showed discriminative power for immunophenotyping of macrophages in a biomimetic tumor environment, which could be potentially applied as a rapid and high throughput method for point-of-care clinical cancer diagnosis.

Techniques: Microarray, Microscopy, Multiplex Assay

Journal: Advanced healthcare materials

Article Title: Magnet Patterned Superparamagnetic Fe 3 O 4 /Au Core-Shell Nanoplasmonic Sensing Array for Label-Free High Throughput Cytokine Immunoassay

doi: 10.1002/adhm.201801478

Figure Lengend Snippet: (a) Schematic of the dark-field microscope setup for FACSNP microarray imaging. The prepared microarray chip was fixed on the motorized stage with the other side in contact with the dark-field condenser via silicon oil. A sample was injected from the inlet of the PDMS channel covered on the prepared pattern, flown through the sample channel, and collected from the outlet. Different cytokines in the sample were captured by the antibody-conjugated FACSNPs on microarray. The light scattered from the FACSNPs was collected by 20X objective lens and imaged by the EMCCD. The right panel shows the principle of the FACSNP microarray imaging. The binding of specific cytokines onto the FACSNP results in an intensity increase and a spectrum red-shift, (b) Mapping of intensity variations of FACSNP microarray for four different types of cytokines (IL-6, MCP-1, TNF-α and TGF-β) at different concentrations. (c) Bar graph showing the scattering intensity variations obtained by the FACSNPs microarray sensing spots within each microfluidic detection channel (horizontal direction) when loaded with one specific recombinant cytokines spiked to the device. The concentration of the specific cytokine in the detection channel is 1000 pg/mL.

Article Snippet: As such, the FACSNP microarray immunoassay showed discriminative power for immunophenotyping of macrophages in a biomimetic tumor environment, which could be potentially applied as a rapid and high throughput method for point-of-care clinical cancer diagnosis.

Techniques: Microscopy, Microarray, Imaging, Injection, Binding Assay, Recombinant, Concentration Assay

Journal: Advanced healthcare materials

Article Title: Magnet Patterned Superparamagnetic Fe 3 O 4 /Au Core-Shell Nanoplasmonic Sensing Array for Label-Free High Throughput Cytokine Immunoassay

doi: 10.1002/adhm.201801478

Figure Lengend Snippet: (a) The schematics of macrophage polarization under different stimulation conditions. The right panel shows the transformation of the macrophages into M2-like TAMs after exposure to a biomimetic tumor microenvironment. (b) Cytokine secretion profiles of macrophages after treated in different polarization conditions. M0 denotes the unpolarized, original macrophages. M1 is the original macrophages treated by 100ng/mL of LPS for 48 hrs. M2 is the original macrophages treated by 100ng/mL of IL-10 for 48 hrs. TAM presents the macrophages polarized by the leukemia tumor condition (LTC) culture medium. The cytokine concentrations were quantified by measuring the cell medium samples (n=3) using the FACSNP microarray immunoassay. Data were presented as mean ± SD. P-values were calculated using the One-Way ANOVA,* P < 0.05, **P<0.01, ***P<0.0001.

Article Snippet: As such, the FACSNP microarray immunoassay showed discriminative power for immunophenotyping of macrophages in a biomimetic tumor environment, which could be potentially applied as a rapid and high throughput method for point-of-care clinical cancer diagnosis.

Techniques: Transformation Assay, Microarray

Journal: PLOS ONE

Article Title: Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

doi: 10.1371/journal.pone.0282233

Figure Lengend Snippet: General characteristics of the studies included in the review.

Article Snippet: DELFIA and Protein microarray immunoassays , S . mansoni , Humans , Serum/plasma , Healthy negative control sera , IgG , Bordier Affinity Products CH and Egg detection. , Italy , 2021 , Stefano De Benedetti [ ] .

Techniques: Control, Microarray, Enzyme-linked Immunosorbent Assay, Negative Control, Luminex, Multiplex Assay, Western Blot